Separation and sensitive detection of D‐amino acids in biological matrices
Abstract
The increase in our understanding of D‐amino acid function and distribution in mammals is in many ways a result of the initial development of sensitive enantioselective separation strategies that allow for quantification in real biological samples. This article reviews progress on the development of chiral selective separation and detection of D‐amino acids including enzyme‐based microbiosensors, GC/MS, HPLC/fluorescence, HPLC/MS‐MS, cEKC/fluorescence, and MEKC/fluorescence. Only methods capable of analyzing D‐isomers in biological matrices are given here and significant effort is made to highlight approaches that offer speed, resolution, high sensitivity, and versatility.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 30, 2009
- Source ID
- 10.1002/jssc.200900101
Entities
People
- Daniel L. Kirschner
- Thomas K. Green
Organizations
- Army Research Office
- National Institute of Neurological Disorders and Stroke
- United States Army Medical Research and Development Command