Comparison of ethylenediaminetetraacetic acid and rapid decalcificier solution for studying human temporal bones by immunofluorescence
Abstract
The pervasiveness of hearing loss and the development of new potential therapeutic approaches have led to increased animal studies of the inner ear. However, translational relevance of such studies depends upon verification of protein localization data in human samples. Cadavers used for anatomical education provide a potential research resource, but are limiting due to difficulties in accessing sensory tissues from the dense temporal bones. This study seeks to reduce the often monthsâlong process of decalcification and improve immunofluorescent staining of human cadaveric temporal bones for research use.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 26, 2020
- Source ID
- 10.1002/lio2.449
Entities
People
- Bradley J Walters
- Mark B. Lewis
- Sumana Ghosh
Organizations
- American Otological Society
- Marine Corps Warfighting Laboratory
- National Institute on Deafness and Other Communication Disorders
- University of Mississippi