Carbohydrate‐Based Polymer Brushes Prevent Viral Adsorption on Electrostatically Heterogeneous Interfaces

Abstract

Chemical heterogeneity on biomaterial surfaces can transform its interfacial properties, rendering nanoscale heterogeneity profoundly consequential during bioadhesion. To examine the role played by chemical heterogeneity in the adsorption of viruses on synthetic surfaces, a range of novel coatings is developed wherein a tunable mixture of electrostatic tethers for viral binding, and carbohydrate brushes, bearing pendant α‐mannose, β‐galactose, or β‐glucose groups, is incorporated. The effects of binding site density, brush composition, and brush architecture on viral adsorption, with the goal of formulating design specifications for virus‐resistant coatings are experimentally evaluated. It is concluded that virus‐coating interactions are shaped by the interplay between brush architecture and binding site density, after quantifying the adsorption of adenoviruses, influenza, and fibrinogen on a library of carbohydrate brushes co‐immobilized with different ratios of binding sites. These insights will be of utility in guiding the design of polymer coatings in realistic settings where they will be populated with defects.

Document Details

Document Type
Pub Defense Publication
Publication Date
Oct 15, 2018
Source ID
10.1002/marc.201800530

Entities

People

  • Domenic Kratzer
  • James Sugai
  • Joerg Lahann
  • Julia Prisby
  • Kenneth Cheng
  • Ramya Kumar
  • William V. Giannobile

Organizations

  • Defense Threat Reduction Agency
  • Karlsruhe Institute of Technology
  • National Science Foundation
  • University of Michigan

Tags

Readers

  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science
  • Surface Coatings Technology.