Templated biological synthesis of polymers of abiological monomers

Abstract

A general method for the in vivo incorporation of amino acid analogues into artificial proteins is described. The method involves the construction of an artificial gene encoding the sequence of interest (with the corresponding natural amino acid encoded in place of the analogue), transformation of a bacterial host strain that cannot synthesize the natural amino acid, and induction of protein synthesis in a host culture enriched in the analogue. Results are described for the amino acid analogues selenomethionine, p‐fluorophenylalanine, trifluoroleucine and 3‐thienylalanine.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jul 01, 1995
Source ID
10.1002/masy.19950980149

Entities

People

  • David A. Tirrell
  • Eiichiro Yoshikawa
  • Maurille J. Fournier
  • Michael J. Dougherty
  • Srinivas Kothakota
  • Thomas L. Mason

Organizations

  • Army Research Office
  • National Science Foundation

Tags

Fields of Study

  • Biology

Readers

  • Computer Engineering
  • Molecular Genetics