NMR quantification of lactate production and efflux and glutamate fractional enrichment in living human prostate biopsies cultured with [1,6‐13C2]glucose
Abstract
Image‐guided prostate biopsies are routinely acquired in the diagnosis and treatment monitoring of prostate cancer, yielding useful tissue for identifying metabolic biomarkers and therapeutic targets. We developed an optimized biopsy tissue culture protocol in combination with [1,6‐13C2]glucose labeling and quantitative high‐resolution NMR to measure glycolysis and tricarboxcylic acid (TCA) cycle activity in freshly acquired living human prostate biopsies.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 28, 2019
- Source ID
- 10.1002/mrm.27739
Entities
People
- Donna M. Peehl
- Hao Nguyen
- Jeremy Bancroft Brown
- Jinny Sun
- John Kurhanewicz
- Justin Delos Santos
- Katsuto Shinohara
- Mark Vancriekinge
- Renuka Sriram
- Romelyn Delos Santos
- Z. Laura Tabatabai
Organizations
- National Cancer Institute
- National Institute of Biomedical Imaging and Bioengineering
- United States Department of Defense
- University of California, San Francisco