Subtype‐specific mutation of PPP2R1A in endometrial and ovarian carcinomas

Abstract

PPP2R1A mutations have recently been described in 3/42 (7%) of clear cell carcinomas of the ovary. PPP2R1A encodes the α‐isoform of the scaffolding subunit of the serine/threonine protein phosphatase 2A (PP2A) holoenzyme. This putative tumour suppressor complex is involved in growth and survival pathways. Through targeted sequencing of PPP2R1A, we identified somatic missense mutations in 40.8% (20/49) of high‐grade serous endometrial tumours, and 5.0% (3/60) of endometrial endometrioid carcinomas. Mutations were also identified in ovarian tumours at lower frequencies: 12.2% (5/41) of endometrioid and 4.1% (2/49) of clear cell carcinomas. No mutations were found in 50 high‐grade and 12 low‐grade serous carcinomas. Amino acid residues affected by these mutations are highly conserved across species and are involved in direct interactions with regulatory B‐subunits of the PP2A holoenzyme. PPP2R1A mutations in endometrial high‐grade serous carcinomas are a frequent and potentially targetable feature of this disease. The finding of frequent PPP2R1A mutations in high‐grade serous carcinoma of the endometrium but not in high‐grade serous carcinoma of the ovary provides clear genetic evidence that these are distinct diseases. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Document Details

Document Type
Pub Defense Publication
Publication Date
Mar 07, 2011
Source ID
10.1002/path.2848

Entities

People

  • Alireza Heravi‐moussavi
  • Anne‐marie Mes‐masson
  • C. Blake Gilks
  • Christine Chow
  • David G. Huntsman
  • Gregg B Morin
  • Janice S Kwon
  • Janine Senz
  • Jessica N Mcalpine
  • Leah M Prentice
  • Mark S. Carey
  • Melissa K Mcconechy
  • Michael S Anglesio
  • Niki Boyd
  • Sohrab P Shah
  • Steve E Kalloger
  • The Australian Ovarian Cancer Study Group
  • Winnie Yang

Organizations

  • United States Army Medical Research and Development Command

Tags

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics
  • Women's Health and Cancer Risk Research: African American Women and Pregnancy Outcomes.

Technology Areas

  • Biotechnology