Enrichment strategies in glycomics‐based lung cancer biomarker development
Abstract
There is a need to identify better glycan biomarkers for diagnosis, early detection, and treatment monitoring in lung cancer using biofluids such as blood. Biofluids are complex mixtures of proteins dominated by a few high abundance proteins that may not have specificity for lung cancer. Therefore, two methods for protein enrichment were evaluated; affinity capturing of IgG and enrichment of medium abundance proteins, thus allowing us to determine which method yields the best candidate glycan biomarkers for lung cancer.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 06, 2013
- Source ID
- 10.1002/prca.201200131
Entities
People
- Ayumu Taguchi
- Carlito B. Lebrilla
- Carol Stroble
- Kyoungmi Kim
- L. Renee Ruhaak
- Samir M Hanash
- Sandra L. Taylor
- Suzanne Miyamoto
- Uyen Thao Nguyen
Organizations
- National Institutes of Health
- United States Department of Defense
- University of California, Davis
- University of Texas at Austin
- Yusuf Hamied Department of Chemistry