De novo design of signal sequences to localize cargo to the 1,2‐propanediol utilization microcompartment
Abstract
Organizing heterologous biosyntheses inside bacterial cells can alleviate common problems owing to toxicity, poor kinetic performance, and cofactor imbalances. A subcellular organelle known as a bacterial microcompartment, such as the 1,2‐propanediol utilization microcompartment of Salmonella, is a promising chassis for this strategy. Here we demonstrate de novo design of the N‐terminal signal sequences used to direct cargo to these microcompartment organelles. We expand the native repertoire of signal sequences using rational and library‐based approaches and show that a canonical leucine‐zipper motif can function as a signal sequence for microcompartment localization. Our strategy can be applied to generate new signal sequences localizing arbitrary cargo proteins to the 1,2‐propanediol utilization microcompartments.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 17, 2017
- Source ID
- 10.1002/pro.3144
Entities
People
- Christopher M. Jakobson
- Danielle Tullman‐ercek
- Marilyn F. Slininger Lee
Organizations
- Army Research Office
- National Science Foundation
- Northwestern University