Solution NMR structure of Se0862, a highly conserved cyanobacterial protein involved in biofilm formation
Abstract
Biofilms are accumulations of microorganisms embedded in extracellular matrices that protect against external factors and stressful environments. Cyanobacterial biofilms are ubiquitous and have potential for treatment of wastewater and sustainable production of biofuels. But the underlying mechanisms regulating cyanobacterial biofilm formation are unclear. Here, we report the solution NMR structure of a protein, Se0862, conserved across diverse cyanobacterial species and involved in regulation of biofilm formation in the cyanobacterium Synechococcus elongatus PCC 7942. Se0862 is a class α+β protein with ααββββαα topology and roll architecture, consisting of a four‐stranded β‐sheet that is flanked by four α‐helices on one side. Conserved surface residues constitute a hydrophobic pocket and charged regions that are likely also present in Se0862 orthologs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 24, 2020
- Source ID
- 10.1002/pro.3952
Entities
People
- Andy LiWang
- Ning Zhang
- Rakefet Schwarz
- Roger Tseng
- Sergey Ovchinnikov
- Yong‐gang Chang
Organizations
- Air Force Office of Scientific Research
- Bar-Ilan University
- Harvard University
- Monash University
- National Science Foundation
- United States Department of Agriculture
- Yusuf Hamied Department of Chemistry