Modeling the structure of pyrococcus furiosus rubredoxin by homology to other X‐ray structures
Abstract
The three‐dimensional structure of rubredoxin from the hyperthermophilic archaebacterium, Pyrococcus furiosus, has been modeled from the X‐ray crystal structures of three homologous proteins from Clostridium pasteurianum, Desulfovibrio gigas, and Desulfovibrio vulgaris. All three homology models are similar. When comparing the positions of all heavy atoms and essential hydrogen atoms to the recently solved crystal structure (Day, M.W., et al., 1992, Protein Sci. 1, 1494–1507) of the same protein, the homology models differ from the X‐ray structure by 2.09 Å root mean square (RMS). The X‐ray and the zinc‐substituted NMR structures (Blake, P.R., et al., 1992b, Protein Sci. 1, 1508–1521) show a similar level of difference (2.05 Å RMS). On average, the homology models are closer to the X‐ray structure than to the NMR structures (2.09 vs. 2.42 Å RMS).
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 1993
- Source ID
- 10.1002/pro.5560020414
Entities
People
- David E. Stewart
- Elizabeth A. Bradley
- John E. Wampler
- Michael W.w. Adams
Organizations
- NATO
- National Institutes of Health
- National Science Foundation
- Office of Naval Research