A comparison of prostate cancer cell transcriptomes in 2D monoculture vs 3D xenografts identify consistent gene expression alterations associated with tumor microenvironments
Abstract
Prostate cancer (PC) research has relied heavily on patient‐derived cell lines, which may be used for in vitro (two‐dimensional [2D]) studies or cultivated as three‐dimensional (3D) xenografts in mice. These approaches are likely to have differential impacts on cell phenotypes, with implications for experimental outcomes. Therefore, defining and comparing the transcriptional signatures associated with 2D and 3D approaches may be useful for designing experiments and interpreting research results.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 18, 2020
- Source ID
- 10.1002/pros.23963
Entities
People
- Clinton K. Matson
- Ilsa M. Coleman
- Lauren Brady
- Michael C. Risk
- Peter S Nelson
- Roger T. Coleman
- Rui M Gil da Costa
Organizations
- Congressionally Directed Medical Research Programs
- Fred Hutchinson Cancer Center
- National Cancer Institute
- University of Minnesota
- University of Washington