3βHSD activity saturates at physiological substrate concentrations in intact cells
Abstract
Conversion of adrenally produced dehydroepiandrosterone (DHEA) to the potent androgen dihydrotestosterone (DHT) is an important mechanism by which prostate cancer reaches castration resistance. At the start of this pathway is a branch point at which DHEA can be converted to Δ4‐androstenedione by the enzyme 3β‐hydroxysteroid dehydrogenase (3βHSD) or to Δ5‐androstenediol by 17βHSD. To better understand this process, we studied the kinetics of these reactions in cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 15, 2023
- Source ID
- 10.1002/pros.24587
Entities
People
- Jeffrey M. Mcmanus
- Nima Sharifi
- Yoon‐Mi Chung
Organizations
- Cleveland Clinic
- Congressionally Directed Medical Research Programs
- Prostate Cancer Foundation
- University of Miami