Characterization of the all‐E. coli transcription‐translation system myTXTL by mass spectrometry
Abstract
Cell‐free transcription‐translation (TXTL) is becoming a popular technology to prototype and engineer biological systems outside living organisms. TXTL relies commonly on a cytoplasmic extract that provides the molecular components necessary to recapitulate gene expression in vitro, where most of the available systems are derived from E. coli. The proteinic and enzymatic composition of lysates, however, is typically unknown. In this work, we analyzed by mass spectrometry the molecular constituents of the all‐E. coli TXTL platform myTXTL prepared from the E. coli strain BL21 Rosetta2.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 14, 2019
- Source ID
- 10.1002/rcm.8438
Entities
People
- Chase L Beisel
- David Garenne
- Vincent Noireaux
Organizations
- Defense Advanced Research Projects Agency
- Human Frontier Science Program
- University of Minnesota
- University of Würzburg