Multiplexed Nucleic Acid Hybridization Assays Using Single‐FRET‐Pair Distance‐Tuning
Abstract
Multiplexed photoluminescence (PL) detection plays an important role in chemical and biological sensing. Here, it is shown that time‐gated (TG) detection of a single terbium‐donor‐based Förster resonance energy transfer (FRET) pair can be used to selectively quantify low nanomolar concentrations of multiple DNAs or microRNAs in a single sample. This study demonstrates the applicability of single‐TG‐FRET‐pair multiplexing for molecular (Tb‐to‐dye) and nanoparticle (Tb‐to‐quantum‐dot) biosensing. Both systems use acceptor‐sensitization and donor‐quenching for quantifying biomolecular recognition and modification of the donor–acceptor distance for tuning the PL decays. TG intensity detection provides extremely low background noise and a quick and simple one‐step assay format. Single‐TG‐FRET‐pair multiplexing can be combined with spectral and spatial resolution, paving the way for biosensing with unprecedented high‐order multiplexing capabilities.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 03, 2017
- Source ID
- 10.1002/smll.201700332
Entities
People
- Alexandra Petreto
- Igor L. Medintz
- Jiajia Guo
- Niko Hildebrandt
- Xue Qiu
- Zongwen Jin
Organizations
- China Scholarship Council
- European Commission
- Institut Universitaire de France
- The Chinese University of Hong Kong
- United States Naval Research Laboratory