Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging
Abstract
A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine perchlorate (DiI), 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indodicarbocyanine perchlorate (DiD), and 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO2‐based technology, show excellent colloidal‐ and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI‐loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 24, 2018
- Source ID
- 10.1002/smll.201703851
Entities
People
- Anna Painelli
- Antonio Ardizzone
- Cristina Sissa
- David J Hagan
- Eric W. Van Stryland
- Jaume Veciana
- Jordi Faraudo
- Lorenzo Albertazzi
- Mykhailo Bondar
- Natalia Feiner
- Nora Ventosa
- Siarhei Kurhuzenkau
- Sílvia Illa‐tuset
Organizations
- AXA Research Fund
- Agency for Administration of University and Research
- Federación Española de Enfermedades Raras
- Institute for Bioengineering of Catalonia
- Institute of Physics
- Jawaharlal Nehru Centre for Advanced Scientific Research
- Ministry of Economy, Industry and Competitiveness
- Seventh Framework Programme
- Spanish National Research Council
- United States Army Research Laboratory
- University of Central Florida