Monocrystalline Labeling Enables Stable Plasmonic Enhancement for Isolation‐Free Extracellular Vesicle Analysis
Abstract
Sensitive detection of extracellular vesicles (EVs) as emerging biomarkers has shown great promises for disease diagnosis. Plasmonic metal nanostructures conjugated with molecules that bind specific biomarker targets are widely used for EVs sensing but involve tradeoffs between particle‐size‐dependent signal intensity and conjugation efficiency. One solution to this problem would be to induce nucleation on nanoparticles that have successfully bound a target biomarker to permit in situ nanoparticle growth for signal amplification, but approaches that are evaluated to date require harsh conditions or lack nucleation specificity, prohibiting their effective use with most biological specimens. This study describes a one‐step in situ strategy to induce monocrystalline copper shell growth on gold nanorod probes without decreasing signal by disrupting probe‐target interactions or lipid bilayer integrity to enable EV biomarker detections. This approach increases the detected nanoparticle signal about two orders of magnitude after a 10 min copper nanoshell growth reaction. This has significant implications for improved disease detection, as indicated by the ability of a novel immunoassay using this approach to detect low abundance EVs carrying a pathogen‐derived biomarker, after their direct capture from serum, to facilitate the diagnosis of tuberculosis cases in a diagnostically challenging pediatric cohort.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 10, 2022
- Source ID
- 10.1002/smll.202204298
Entities
People
- Chen‐zhong Li
- Christopher J. Lyon
- Julian G. Saliba
- Lili Zhang
- Ruixuan Wang
- Shu Wang
- Sutapa Chandra
- Tao Wang
- Tony Y. Hu
- Wenshu Zheng
- Yang Li
Organizations
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- National Institute of Allergy and Infectious Diseases
- National Institute of Neurological Disorders and Stroke
- Tulane University of Louisiana
- United States Department of Defense