Estimating the proportions in a mixed sample using transcriptomics
Abstract
Often, biomedical researchers take a tissue sample from a tumour that unintentionally contains tumour cells and stromal cells so that gene expression from the sample represents two distinct kinds of cells. This is tolerated because techniques for separating tumour and stromal cells are expensive and time‐consuming and the act of separation can alter gene expression. So, it is desirable to have a technique for estimating the proportion of tumour cells in a mixed sample to improve detection of differential expression in cancer cells. © 2014 The Authors. Stat Published by John Wiley & Sons Ltd.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 01, 2014
- Source ID
- 10.1002/sta4.65
Entities
People
- Bertrand Clarke
- Jennifer Clarke
Organizations
- Defense Threat Reduction Agency
- National Science Foundation
- University of Nebraska–Lincoln