Recent advancement of light‐based single‐molecule approaches for studying biomolecules

Abstract

Recent advances in single‐molecule techniques have led to new discoveries in analytical chemistry, biophysics, and medicine. Understanding the structure and behavior of single biomolecules provides a wealth of information compared to studying large ensembles. However, developing single‐molecule techniques is challenging and requires advances in optics, engineering, biology, and chemistry. In this paper, we will review the state of the art in single‐molecule applications with a focus over the last few years of development. The advancements covered will mainly include light‐based in vitro methods, and we will discuss the fundamentals of each with a focus on the platforms themselves. We will also summarize their limitations and current and future applications to the wider biological and chemical fields.

Document Details

Document Type
Pub Defense Publication
Publication Date
Feb 06, 2019
Source ID
10.1002/wsbm.1445

Entities

People

  • Benjamin Croop
  • Chenyi Zhang
  • Kyu Young Han
  • Ryan M. Gelfand
  • Youngbin Lim

Organizations

  • Defense Advanced Research Projects Agency
  • Division of Chemical, Bioengineering, Environmental, and Transport Systems
  • Stanford University
  • University of Central Florida

Tags

Fields of Study

  • Chemistry

Readers

  • Academic Conference Management
  • Molecular and Cellular Biochemistry
  • Systems Analysis and Design

Technology Areas

  • Biotechnology