Cell-free systems for accelerating glycoprotein expression and biomanufacturing
Abstract
Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar moieties, plays critical roles in cellular function, human health, and biotechnology. However, studying and producing defined glycoproteins remains challenging. Cell-free glycoprotein synthesis systems, in which protein synthesis and glycosylation are performed in crude cell extracts, offer new approaches to address these challenges. Here, we review versatile, state-of-the-art systems for biomanufacturing glycoproteins in prokaryotic and eukaryotic cell-free systems with natural and synthetic N-linked glycosylation pathways. We discuss existing challenges and future opportunities in the use of cell-free systems for the design, manufacture, and study of glycoprotein biomedicines.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 01, 2020
- Source ID
- 10.1007/s10295-020-02321-4
Entities
People
- Jasmine M Hershewe
- Michael C Jewett
- Weston Kightlinger
Organizations
- Air Force Research Laboratory
- Army Research Office
- David and Lucile Packard Foundation
- Defense Advanced Research Projects Agency
- Defense Threat Reduction Agency
- Gates Foundation
- National Institutes of Health
- Northwestern University