Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus
Abstract
Like many other aerobic archaea, the hyperthermophile Sulfolobus solfataricus possesses a gene cluster encoding components of a putative 2‐oxoacid dehydrogenase complex. In the current paper, we have cloned and expressed the first two genes of this cluster and demonstrate that the protein products form an α2β2 hetero‐tetramer possessing the catalytic activity characteristic of the first component enzyme of an acetoin dehydrogenase multienzyme complex. This represents the first report of an acetoin multienzyme complex in archaea, and contrasts with the branched‐chain 2‐oxoacid dehydrogenase complex activities characterised in two other archaea, Thermoplasma acidophilum and Haloferax volcanii.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 18, 2010
- Source ID
- 10.1016/j.febslet.2010.02.037
Entities
People
- David W. Hough
- Karl A.p. Payne
- Michael J. Danson
Organizations
- Air Force Office of Scientific Research