Pyrrolysyl‐tRNA synthetase variants reveal ancestral aminoacylation function
Abstract
Pyrrolysyl‐tRNA synthetase (PylRS) is a class IIc aminoacyl‐tRNA synthetase that is related to phenylalanyl‐tRNA synthetase (PheRS). Genetic selection provided PylRS variants with a broad range of specificity for diverse non‐canonical amino acids (ncAAs). One variant is a specific phenylalanine‐incorporating enzyme. Structural models of the PylRSamino acid complex show that the small pocket size and π‐interaction play an important role in specific recognition of Phe and the engineered PylRS active site resembles that of Escherichia coli PheRS.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 27, 2013
- Source ID
- 10.1016/j.febslet.2013.08.018
Entities
People
- Akiyoshi Nakamura
- Dieter Söll
- Jae-hyeong Ko
- Li-tao Guo
- Takuya Umehara
- Yane-Shih Wang
Organizations
- Defense Advanced Research Projects Agency
- National Institute of General Medical Sciences