Epigenetic reprogramming of cell cycle genes by ACK1 promotes breast cancer resistance to CDK4/6 inhibitor
Abstract
Hormone receptor-positive, HER2-negative advanced breast cancers exhibit high sensitivity to CDK4/6 inhibitors such as palbociclib. However, most patients inevitably develop resistance, thus identification of new actionable therapeutic targets to overcome the recurrent disease is an urgent need. Immunohistochemical studies of tissue microarray revealed increased activation of non-receptor tyrosine kinase, ACK1 (also known as TNK2) in most of the breast cancer subtypes, independent of their hormone receptor status. Chromatin immunoprecipitation studies demonstrated that the nuclear target of activated ACK1, pY88-H4 epigenetic marks, were deposited at cell cycle genes,CCNB1, CCNB2andCDC20, which in turn initiated their efficient transcription. Pharmacological inhibition of ACK1 using its inhibitor, (R)-9bdampenedCCNB1, CCNB2andCDC20expression, caused G2/M arrest, culminating in regression of palbociclib-resistant breast tumor growth. Further, (R)-9bsuppressed expression of CXCR4 receptor, which resulted in significant impairment of metastasis of breast cancer cells to lung. Overall, our pre-clinical data identifies activated ACK1 as an oncogene that epigenetically controls the cell cycle genes governing the G2/M transition in breast cancer cells. ACK1 inhibitor, (R)-9bcould be a novel therapeutic option for the breast cancer patients that have developed resistance to CDK4/6 inhibitors.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 17, 2023
- Source ID
- 10.1038/s41388-023-02747-x
Entities
People
- Akhilesh Pandey
- Audrey Wilson
- Christopher J. O’conor
- Cody Weimholt
- Dhivya Sridaran
- Ian S Hagemann
- Jingqin Luo
- Juan Carlos Roa
- Kiran Mahajan
- Mithila Sawant
- Nupam P. Mahajan
- Tiandao Li
- Xinyan Wu
Organizations
- Division of Cancer Epidemiology and Genetics, National Cancer Institute
- National Cancer Institute
- United States Department of Defense