Defective transcription elongation in a subset of cancers confers immunotherapy resistance

Abstract

The nature and role of global transcriptional deregulations in cancers are not fully understood. We report that a large proportion of cancers have widespread defects in mRNA transcription elongation (TE). Cancers with TE defects (TEdeff) display spurious transcription and defective mRNA processing of genes characterized by long genomic length, poised promoters and inducible expression. Signaling pathways regulated by such genes, such as pro-inflammatory response pathways, are consistently suppressed in TEdeff tumors. Remarkably, TEdeff correlates with the poor response and outcome in immunotherapy, but not chemo- or targeted therapy, -treated renal cell carcinoma and metastatic melanoma patients. Forced pharmacologic or genetic induction of TEdeff in tumor cells impairs pro-inflammatory response signaling, and imposes resistance to the innate and adaptive anti-tumor immune responses and checkpoint inhibitor therapy in vivo. Therefore, defective TE is a previously unknown mechanism of tumor immune resistance, and should be assessed in cancer patients undergoing immunotherapy.

Document Details

Document Type
Pub Defense Publication
Publication Date
Oct 23, 2018
Source ID
10.1038/s41467-018-06810-0

Entities

People

  • Belal Muhammad
  • Edith M. Janssen
  • Eunah Chung
  • Gang Huang
  • Joo-Seop Park
  • Kakajan Komurov
  • Kashish Chetal
  • Kwangmin Choi
  • Lisa M Privette Vinnedge
  • Matthew T Weirauch
  • Nancy Ratner
  • Nathan Salomonis
  • Navneet Singh
  • Susan E. Waltz
  • Vakul Mohanty
  • Vishnu Modur
  • Xiaoting Chen

Organizations

  • National Cancer Institute
  • United States Department of Defense

Tags

Fields of Study

  • Biology
  • Medicine

Readers

  • Molecular Biology and Genetics
  • Molecular and Cellular Biology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech