Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
Abstract
Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 04, 2019
- Source ID
- 10.1038/s41467-019-10534-0
Entities
People
- Aaron R Halpern
- Adam K Glaser
- Caleb Stoltzfus
- Chengbo Yin
- Chenyi Mao
- Erin F. Mccarty
- Jonathan S. Daniels
- Jonathan T C Liu
- Joshua C. Vaughan
- Lawrence D. True
- Lindsey A. Barner
- Linpeng Wei
- Michael Y Gerner
- Nicholas P. Reder
- Philip R. Nicovich
- Soyoung Kang
- Weisi Xie
- Ye Chen
Organizations
- National Institutes of Health
- Prostate Cancer Foundation
- United States Department of Defense