DNA assembly for nanopore data storage readout
Abstract
Synthetic DNA is becoming an attractive substrate for digital data storage due to its density, durability, and relevance in biological research. A major challenge in making DNA data storage a reality is that reading DNA back into data using sequencing by synthesis remains a laborious, slow and expensive process. Here, we demonstrate successful decoding of 1.67 megabytes of information stored in short fragments of synthetic DNA using a portable nanopore sequencing platform. We design and validate an assembly strategy for DNA storage that drastically increases the throughput of nanopore sequencing. Importantly, this assembly strategy is generalizable to any application that requires nanopore sequencing of small DNA amplicons.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 03, 2019
- Source ID
- 10.1038/s41467-019-10978-4
Entities
People
- Georg Seelig
- Karin Strauss
- Konstantin Makarychev
- Luis Ceze
- Miklós Z. Rácz
- Randolph Lopez
- Sergey Yekhanin
- Siena Dumas Ang
- Yuan-Jyue Chen
Organizations
- Microsoft
- United States Department of Defense