Tsc1 represses parvalbumin expression and fast-spiking properties in somatostatin lineage cortical interneurons

Abstract

Medial ganglionic eminence (MGE)-derived somatostatin (SST)+ and parvalbumin (PV)+ cortical interneurons (CINs), have characteristic molecular, anatomical and physiological properties. However, mechanisms regulating their diversity remain poorly understood. Here, we show that conditional loss of the Tuberous Sclerosis Complex (TSC) gene, Tsc1, which inhibits the mammalian target of rapamycin (MTOR), causes a subset of SST+ CINs, to express PV and adopt fast-spiking (FS) properties, characteristic of PV+ CINs. Milder intermediate phenotypes also occur when only one allele of Tsc1 is deleted. Notably, treatment of adult mice with rapamycin, which inhibits MTOR, reverses the phenotypes. These data reveal novel functions of MTOR signaling in regulating PV expression and FS properties, which may contribute to TSC neuropsychiatric symptoms. Moreover, they suggest that CINs can exhibit properties intermediate between those classically associated with PV+ or SST+ CINs, which may be dynamically regulated by the MTOR signaling.

Document Details

Document Type
Pub Defense Publication
Publication Date
Nov 01, 2019
Source ID
10.1038/s41467-019-12962-4

Entities

People

  • Anna Noren Rubin
  • April M Stafford
  • Daniel Vogt
  • Emily Ling-Lin Pai
  • John L. Rubenstein
  • Kartik Angara
  • Petros Minasi
  • Ruchi Malik
  • Vikaas S. Sohal

Organizations

  • Brain & Behavior Research Foundation
  • Congressionally Directed Medical Research Programs
  • National Institute of Mental Health

Tags

Fields of Study

  • Biology

Readers

  • Aquatic Ecology
  • Asian Economic Studies
  • Neuroscience