Imaging brain activity during complex social behaviors in Drosophila with Flyception2

Abstract

Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male flies. We discover that P1 neurons, active during courtship, are inactive during copulation, whereas GABAergic mAL neurons remain active during copulation, suggesting a countervailing role of mAL in opposing P1 activity during mating.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 30, 2020
Source ID
10.1038/s41467-020-14487-7

Entities

People

  • Dhruv Grover
  • Jinfang Li
  • Ralph J. Greenspan
  • Takeo Katsuki
  • Thomas J. Dawkins

Organizations

  • Air Force Office of Scientific Research

Tags

Readers

  • Gender and Food Studies
  • Neuroscience
  • Robotics and Automation.