Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard
Abstract
DNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 13, 2020
- Source ID
- 10.1038/s41467-020-18910-x
Entities
People
- Alexander Auer
- Anne Louise Kodal
- Bhavik Nathwani
- Florian Schueder
- Jaeseung Hahn
- Jianghong Min
- Johannes B. Woehrstein
- Jörg Schnitzbauer
- Maartje M. Bastings
- Maximilian T. Strauss
- Nandhini Ponnuswamy
- Peng Yin
- Ralf Jungmann
- Sarah W. Helmig
- Seungwoo Lee
- Shelley F. J. Wickham
- Steven D. Perrault
- William M Shih
- Zhao Zhao
Organizations
- Army Research Office