Shortwave-infrared meso-patterned imaging enables label-free mapping of tissue water and lipid content
Abstract
Water and lipids are key participants in many biological processes, but there are few non-invasive methods that provide quantification of these components in vivo, and none that can isolate and quantify lipids in the blood. Here we develop a new imaging modality termed shortwave infrared meso-patterned imaging (SWIR-MPI) to provide label-free, non-contact, spatial mapping of water and lipid concentrations in tissue. The method utilizes patterned hyperspectral illumination to target chromophore absorption bands in the 900–1,300 nm wavelength range. We use SWIR-MPI to monitor clinically important physiological processes including edema, inflammation, and tumor lipid heterogeneity in preclinical models. We also show that SWIR-MPI can spatially map blood-lipids in humans, representing an example of non-invasive and contact-free measurements of in vivo blood lipids. Together, these results highlight the potential of SWIR-MPI to enable new capabilities in fundamental studies and clinical monitoring of major conditions including obesity, cancer, and cardiovascular disease.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 23, 2020
- Source ID
- 10.1038/s41467-020-19128-7
Entities
People
- Anahita Pilvar
- Anup Tank
- Darren Roblyer
- Hannah Peterson
- John Jiang
- John P Dumas
- Jon C. Aster
- Mark C. Pierce
- Yanyu Zhao
Organizations
- United States Department of Defense