Digital plasmonic nanobubble detection for rapid and ultrasensitive virus diagnostics
Abstract
Rapid and sensitive diagnostics of infectious diseases is an urgent and unmet need as evidenced by the COVID-19 pandemic. Here, we report a strategy, based on DIgitAl plasMONic nanobubble Detection (DIAMOND), to address this need. Plasmonic nanobubbles are transient vapor bubbles generated by laser heating of plasmonic nanoparticles (NPs) and allow single-NP detection. Using gold NPs as labels and an optofluidic setup, we demonstrate that DIAMOND achieves compartment-free digital counting and works on homogeneous immunoassays without separation and amplification steps. DIAMOND allows specific detection of respiratory syncytial virus spiked in nasal swab samples and achieves a detection limit of ~100 PFU/mL (equivalent to 1 RNA copy/µL), which is competitive with digital isothermal amplification for virus detection. Therefore, DIAMOND has the advantages including one-step and single-NP detection, direct sensing of intact viruses at room temperature, and no complex liquid handling, and is a platform technology for rapid and ultrasensitive diagnostics.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 30, 2022
- Source ID
- 10.1038/s41467-022-29025-w
Entities
People
- Chen Xie
- Haihang Ye
- Hoangdinh Huynh
- Jeffrey S. Kahn
- Peiyuan Kang
- Yaning Liu
- Zhenpeng Qin
Organizations
- Congressionally Directed Medical Research Programs
- National Institute of Allergy and Infectious Diseases
- United States Department of Health and Human Services