Real-time 3D analysis during electron tomography using tomviz
Abstract
The demand for high-throughput electron tomography is rapidly increasing in biological and material sciences. However, this 3D imaging technique is computationally bottlenecked by alignment and reconstruction which runs from hours to days. We demonstrate real-time tomography with dynamic 3D tomographic visualization to enable rapid interpretation of specimen structure immediately as data is collected on an electron microscope. Using geometrically complex chiral nanoparticles, we show volumetric interpretation can begin in less than 10 minutes and a high-quality tomogram is available within 30 minutes. Real-time tomography is integrated into tomviz, an open-source and cross-platform 3D data analysis tool that contains intuitive graphical user interfaces (GUI), to enable any scientist to characterize biological and material structure in 3D.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 01, 2022
- Source ID
- 10.1038/s41467-022-32046-0
Entities
People
- Alessandro Genova
- Anastasiia Visheratina
- Berk Geveci
- Brianna Major
- Chris Harris
- David A. Muller
- Huihuo Zheng
- Jacob Pietryga
- Jonathan Schwartz
- Marcus Hanwell
- Nicholas A. Kotov
- Patrick Avery
- Peter Ercius
- Prashant Kumar
- Robert Hovden
- Utkarsh Ayachit
- Yi Jiang
Organizations
- Army Research Office