In-depth mapping of protein localizations in whole tissue by micro-scaffold assisted spatial proteomics (MASP)
Abstract
Accurate, in-depth mapping of proteins on whole-tissue levels provides comprehensive insights into the spatially-organized regulatory processes/networks in tissues, but is challenging. Here we describe a micro-scaffold assisted spatial proteomics (MASP) strategy, based on spatially-resolved micro-compartmentalization of tissue using a 3D-printed micro-scaffold, capable of mapping thousands of proteins across a whole-tissue slice with excellent quantitative accuracy/precision. The pipeline includes robust tissue micro-compartmentalization with precisely-preserved spatial information, reproducible procurement and preparation of the micro-specimens, followed by sensitive LC-MS analysis and map generation by a MAsP app. The mapping accuracy was validated by comparing the MASP-generated maps of spiked-in peptides and brain-region-specific markers with known patterns, and by correlating the maps of the two protein components of the same heterodimer. The MASP was applied in mapping >5000 cerebral proteins in the mouse brain, encompassing numerous important brain markers, regulators, and transporters, where many of these proteins had not previously been mapped on the whole-tissue level.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 14, 2022
- Source ID
- 10.1038/s41467-022-35367-2
Entities
People
- Bo An
- Chao Xue
- Jianmin Wang
- Jie Pu
- Jun Qu
- Min Ma
- Ming Zhang
- Sailee Rasam
- Shichen Shen
- Shihan Huo
- Shuo Qian
- Xiaoyu Zhu
Organizations
- National Cancer Institute
- National Heart, Lung, and Blood Institute
- National Institute of Diabetes and Digestive and Kidney Diseases
- National Institute on Aging
- United States Department of Defense