Stressed target cancer cells drive nongenetic reprogramming of CAR T cells and solid tumor microenvironment
Abstract
The poor efficacy of chimeric antigen receptor T-cell therapy (CAR T) for solid tumors is due to insufficient CAR T cell tumor infiltration, in vivo expansion, persistence, and effector function, as well as exhaustion, intrinsic target antigen heterogeneity or antigen loss of target cancer cells, and immunosuppressive tumor microenvironment (TME). Here we describe a broadly applicable nongenetic approach that simultaneously addresses the multiple challenges of CAR T as a therapy for solid tumors. The approach reprograms CAR T cells by exposing them to stressed target cancer cells which have been exposed to the cell stress inducer disulfiram (DSF) and copper (Cu)(DSF/Cu) plus ionizing irradiation (IR). The reprogrammed CAR T cells acquire early memory-like characteristics, potent cytotoxicity, enhanced in vivo expansion, persistence, and decreased exhaustion. Tumors stressed by DSF/Cu and IR also reprogram and reverse the immunosuppressive TME in humanized mice. The reprogrammed CAR T cells, derived from peripheral blood mononuclear cells of healthy donors or metastatic female breast cancer patients, induce robust, sustained memory and curative anti-solid tumor responses in multiple xenograft mouse models, establishing proof of concept for empowering CAR T by stressing tumor as a promising therapy for solid tumors.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 15, 2023
- Source ID
- 10.1038/s41467-023-41282-x
Entities
People
- Albert B. Deleo
- Allison M. Kehlmann
- Alphonse G. Taghian
- Cheng Wang
- Cristina Maccalli
- Cristina R. Ferrone
- David L. Drum
- Emre Dal
- Feng Chen
- Fengfei Sun
- Fubao Liu
- Genevieve Boland
- Gianpietro Dotti
- Giulia Cattaneo
- Hanyu Zhang
- Hui Zheng
- Jibing Yang
- Jingyu Jia
- Laiping Wong
- Lin Jia
- Ling Yu
- Marco Ventin
- Mingliang Wang
- Peigen Huang
- Ruochuan Sun
- Ruslan I Sadreyev
- Shahrzad Arya
- Soldano Ferrone
- Song Fan
- Steven J. Isakoff
- Xinhui Wang
- Yida Zhang
- Youssef Jounaidi
- Yufeng Wang
Organizations
- National Institutes of Health
- United States Department of Defense
- United States Department of Health and Human Services