LRRC10 regulates mammalian cardiomyocyte cell cycle during heart regeneration
Abstract
Leucine-rich repeat containing 10 (LRRC10) is a cardiomyocyte-specific protein, but its role in cardiac biology is little understood. Recently Lrrc10 was identified as required for endogenous cardiac regeneration in zebrafish; however, whether LRRC10 plays a role in mammalian heart regeneration remains unclear. In this study, we demonstrate that Lrrc10–/– knockout mice exhibit a loss of the neonatal mouse regenerative response, marked by reduced cardiomyocyte cytokinesis and increased cardiomyocyte binucleation. Interestingly, LRRC10 deletion disrupts the regenerative transcriptional landscape of the regenerating neonatal mouse heart. Remarkably, cardiac overexpression of LRRC10 restores cardiomyocyte cytokinesis, increases cardiomyocyte mononucleation, and the cardiac regenerative capacity of Lrrc10–/– mice. Our results are consistent with a model in which LRRC10 is required for cardiomyocyte cytokinesis as well as regulation of the transcriptional landscape during mammalian heart regeneration.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 28, 2023
- Source ID
- 10.1038/s41536-023-00316-0
Entities
People
- Ahmed I Mahmoud
- Alyssa R. Schuett
- Dakota J. Nuttall
- Jiyoung Bae
- Kayla N. Wanless
- Megan C. Mckeon
- Rebecca J. Salamon
- Rupa Sridharan
- Stephen A. Nemr
- Timothy J. Kamp
- Wyatt G. Paltzer
- Xiaoya Zhang
- Youngsook Lee
Organizations
- American Heart Association
- National Heart, Lung, and Blood Institute
- National Institute of General Medical Sciences
- National Science Foundation
- United States Department of Defense
- United States Department of Health and Human Services
- University of Wisconsin–Madison