Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice
Abstract
Development of SARS-CoV-2 vaccines that protect vulnerable populations is a public health priority. Here, we took a systematic and iterative approach by testing several adjuvants and SARS-CoV-2 antigens to identify a combination that elicits antibodies and protection in young and aged mice. While demonstrating superior immunogenicity to soluble receptor-binding domain (RBD), RBD displayed as a protein nanoparticle (RBD-NP) generated limited antibody responses. Comparison of multiple adjuvants including AddaVax, AddaS03, and AS01B in young and aged mice demonstrated that an oil-in-water emulsion containing carbohydrate fatty acid monosulphate derivative (CMS:O/W) most effectively enhanced RBD-NP-induced cross-neutralizing antibodies and protection across age groups. CMS:O/W enhanced antigen retention in the draining lymph node, induced injection site, and lymph node cytokines, with CMS inducing MyD88-dependent Th1 cytokine polarization. Furthermore, CMS and O/W synergistically induced chemokine production from human PBMCs. Overall, CMS:O/W adjuvant may enhance immunogenicity and protection of vulnerable populations against SARS-CoV-2 and other infectious pathogens.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 14, 2023
- Source ID
- 10.1038/s41541-023-00610-4
Entities
People
- Aaron G. Schmidt
- Aiquan Chang
- Al Ozonoff
- Andrew Z. Xu
- Blake M Hauser
- Carly Dillen
- Dan H. Barouch
- Danica Dong
- David J. Dowling
- Etsuro Nanishi
- Francesco Borriello
- Hyuk-Soo Seo
- Ivan Zanoni
- Jared Feldman
- Jing Chen
- Jingyou Yu
- Joann Diray-arce
- Kijun Song
- Lindsey R. Baden
- Luuk Hilgers
- Manisha Menon
- Marisa E McGrath
- Matthew B. Frieman
- Ofer Levy
- Peter Paul Platenburg
- Robert K Ernst
- Sirano Dhe-paganon
- Soumik Barman
- Timothy M Caradonna
- Timothy R. O’Meara
- Yoshine Saito
Organizations
- Biomedical Advanced Research and Development Authority
- National Institute of Allergy and Infectious Diseases
- National Institutes of Health
- United States Department of Defense
- United States Department of Health and Human Services