Signature maps for automatic identification of prostate cancer from colorimetric analysis of H&E- and IHC-stained histopathological specimens
Abstract
Prostate cancer (PCa) is a major cause of cancer death among men. The histopathological examination of post-surgical prostate specimens and manual annotation of PCa not only allow for detailed assessment of disease characteristics and extent, but also supply the ground truth for developing of computer-aided diagnosis (CAD) systems for PCa detection before definitive treatment. As manual cancer annotation is tedious and subjective, there have been a number of publications describing methods for automating the procedure via the analysis of digitized whole-slide images (WSIs). However, these studies have focused only on the analysis of WSIs stained with hematoxylin and eosin (H&E), even though there is additional information that could be obtained from immunohistochemical (IHC) staining. In this work, we propose a framework for automating the annotation of PCa that is based on automated colorimetric analysis of both H&E and IHC WSIs stained with a triple-antibody cocktail against high-molecular weight cytokeratin (HMWCK), p63, and α-methylacyl CoA racemase (AMACR). The analysis outputs were then used to train a regression model to estimate the distribution of cancerous epithelium within slides. The approach yielded an AUC of 0.951, sensitivity of 87.1%, and specificity of 90.7% as compared to slide-level annotations, and generalized well to cancers of all grades.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 06, 2019
- Source ID
- 10.1038/s41598-019-43486-y
Entities
People
- Andrew D. Johnson
- Anthony E. Rizzardi
- Benjamin M. Brassuer
- Ethan Leng
- Gregory J. Metzger
- Jin Jin
- Jonathan C. Henriksen
- Joseph S Koopmeiners
- Jung Who Nam
- Nicholas P. Reder
- Stephen C. Schmechel
Organizations
- Congressionally Directed Medical Research Programs
- Graduate School, University of Minnesota
- National Cancer Institute
- National Center for Advancing Translational Sciences
- National Institute of Biomedical Imaging and Bioengineering
- National Institute of General Medical Sciences