Burn-related Collagen Conformational Changes in ex vivo Porcine Skin using Raman Spectroscopy
Abstract
This study utilizes Raman spectroscopy to analyze the burn-induced collagen conformational changes in ex vivo porcine skin tissue. Raman spectra of wavenumbers 500–2000 cm−1 were measured for unburnt skin as well as four different burn conditions: (i) 200 °F for 10 s, (ii) 200 °F for the 30 s, (iii) 450 °F for 10 s and (iv) 450 °F for 30 s. The overall spectra reveal that protein and amino acids-related bands have manifested structural changes including the destruction of protein-related functional groups, and transformation from α-helical to disordered structures which are correlated with increasing burn severity. The deconvolution of the amide I region (1580–1720 cm−1) and the analysis of the sub-bands reveal a change of the secondary structure of the collagen from the α-like helix dominated to the β-aggregate dominated one. Such conformational changes may explain the softening of mechanical response in burnt tissues reported in the literature.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 16, 2019
- Source ID
- 10.1038/s41598-019-55012-1
Entities
People
- Hanglin Ye
- Jack Norfleet
- Rahul
- Su-Fei Shi
- Suvranu De
- Tianmeng Wang
- Uwe Kruger
Organizations
- United States Army Research Laboratory