Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury
Abstract
Traumatic brain injury (TBI) is linked to long-term symptoms in a sub-set of patients who sustain an injury, but this risk is not universal, leading us and others to question the nature of individual variability in recovery trajectories. Extracellular vesicles (EVs) are a promising, novel avenue to identify blood-based biomarkers for TBI. Here, our aim was to determine if glial fibrillary acidic protein (GFAP) and neurofilament light (NfL) measured 1-year postinjury in EVs could distinguish patients from controls, and whether these biomarkers relate to TBI severity or recovery outcomes. EV GFAP and EV NfL were measured using an ultrasensitive assay in 72 TBI patients and 20 controls. EV GFAP concentrations were elevated in moderate and severe TBI compared to controls (p’s p’s < 0.05). These findings suggest that blood-derived EV concentrations of GFAP and NfL drawn even 1 year after injury are higher in TBI patients compared to controls, and are related to injury severity and poor recovery outcomes, suggesting that TBIs alter the activity of these biomarkers, likely contributing to individual variability in recovery.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 16, 2021
- Source ID
- 10.1038/s41598-021-82875-0
Entities
People
- Andre Van Der Merwe
- Bao-xi Qu
- Brian E. Moore
- Cassandra Pattinson
- Chen Lai
- Christina Devoto
- Jacqueline Leete
- Jessica Gill
- Kisha Greer
- Leighton Chan
- Pashtun Shahim
- Spencer Flynn
- Vindhya Ekanayake
- Vivian A. Guedes
Organizations
- Center for Neuroscience and Regenerative Medicine
- National Institutes of Health