Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor
Abstract
Oxygenation is a crucial indicator of tissue viability and function. Oxygen tension ($$\hbox {pO}_2$$ pO 2 ), i.e. the amount of molecular oxygen present in the tissue is a direct result of supply (perfusion) and consumption. Thus, measurement of $$\hbox {pO}_{{2}}$$ pO 2 is an effective method to monitor tissue viability. However, tissue oximetry sensors commonly used in clinical practice instead rely on measuring oxygen saturation ($$\hbox {StO}_2$$ StO 2 ), largely due to the lack of reliable, affordable $$\hbox {pO}_2$$ pO 2 sensing solutions. To address this issue we present a proof-of-concept design and validation of a low-cost, lifetime-based oxygen sensing fiber. The sensor consists of readily-available off-the shelf components such as a microcontroller, a light-emitting diode (LED), an avalanche photodiode (APD), a temperature sensor, as well as a bright in-house developed porphyrin molecule. The device was calibrated using a benchtop setup and evaluated in three in vivo animal models. Our findings show that the new device design in combination with the bright porphyrin has the potential to be a useful and accurate tool for measuring $$\hbox {pO}_2$$ pO 2 in tissue, while also highlighting some of the limitations and challenges of oxygen measurements in this context.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 07, 2023
- Source ID
- 10.1038/s41598-023-41917-5
Entities
People
- Alexis Cralley
- Avery Goss
- Bing Li
- Christene A Huang
- Conor L Evans
- Dor Yoeli
- Emmanuel Roussakis
- Ernest Moore
- Hunter B. Moore
- Juan Pedro Cascales
- Lilian Witthauer
- Xiaolei Li
- Yong Wang
- Zhaohui Wang
Organizations
- United States Department of Defense