AePUb promoter length modulates gene expression in Aedes aegypti
Abstract
Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 21, 2023
- Source ID
- 10.1038/s41598-023-47777-3
Entities
People
- Leonela Carabajal Paladino
- Luke Alphey
- Michelle A E Anderson
- Philip T. Leftwich
- Ray Wilson
- Sanjay Basu
- Sara Rooney
- Zach N. Adelman
Organizations
- Biotechnology and Biological Sciences Research Council
- Defense Advanced Research Projects Agency
- Wellcome