Extraction and biomolecular analysis of dermal interstitial fluid collected with hollow microneedles
Abstract
Dermal interstitial fluid (ISF) is an underutilized information-rich biofluid potentially useful in health status monitoring applications whose contents remain challenging to characterize. Here, we present a facile microneedle approach for dermal ISF extraction with minimal pain and no blistering for human subjects and rats. Extracted ISF volumes were sufficient for determining transcriptome, and proteome signatures. We noted similar profiles in ISF, serum, and plasma samples, suggesting that ISF can be a proxy for direct blood sampling. Dynamic changes in RNA-seq were recorded in ISF from induced hypoxia conditions. Finally, we report the first isolation and characterization, to our knowledge, of exosomes from dermal ISF. The ISF exosome concentration is 12–13 times more enriched when compared to plasma and serum and represents a previously unexplored biofluid for exosome isolation. This minimally invasive extraction approach can enable mechanistic studies of ISF and demonstrates the potential of ISF for real-time health monitoring applications.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 22, 2018
- Source ID
- 10.1038/s42003-018-0170-z
Entities
People
- Anupama Sinha
- Bao Quoc Tran
- Gabrielle Boyd
- Justin T Baca
- Kelly P Williams
- Kunal Poorey
- Philip R. Miller
- Raga Krishnakumar
- Robert M. Taylor
- Ronen Polsky
- Steven S. Branda
- Trevor Glaros
- Victor H. Chavez
Organizations
- Defense Threat Reduction Agency