Interferon signaling promotes tolerance to chromosomal instability during metastatic evolution in renal cancer

Abstract

Molecular routes to metastatic dissemination are critical determinants of aggressive cancers. Through in vivo CRISPR–Cas9 genome editing, we generated somatic mosaic genetically engineered models that faithfully recapitulate metastatic renal tumors. Disruption of 9p21 locus is an evolutionary driver to systemic disease through the rapid acquisition of complex karyotypes in cancer cells. Cross-species analysis revealed that recurrent patterns of copy number variations, including 21q loss and dysregulation of the interferon pathway, are major drivers of metastatic potential. In vitro and in vivo genomic engineering, leveraging loss-of-function studies, along with a model of partial trisomy of chromosome 21q, demonstrated a dosage-dependent effect of the interferon receptor genes cluster as an adaptive mechanism to deleterious chromosomal instability in metastatic progression. This work provides critical knowledge on drivers of renal cell carcinoma progression and defines the primary role of interferon signaling in constraining the propagation of aneuploid clones in cancer evolution.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jun 26, 2023
Source ID
10.1038/s43018-023-00584-1

Entities

People

  • A Ari Hakimi
  • Alaa M. T. Mohamed
  • Alessandro Carugo
  • Alessandro Sgambato
  • Andrea Viale
  • Christopher A. Bristow
  • Cihui Zhu
  • Courtney N. Le
  • Edoardo Del Poggetto
  • Eliezer Van Allen
  • Eric Jonasch
  • Federica Carbone
  • Francesca Citron
  • Giannicola Genovese
  • Giulio F Draetta
  • Hania Khan
  • Hideo Tomihara
  • Jianhua Zhang
  • Jianjun Gao
  • Justin K. Huang
  • Kanishka Sircar
  • Kevin Litchfield
  • Li Zhang
  • Linghua Wang
  • Luigi Perelli
  • Melinda Soeung
  • Michael Peoples
  • Nizar M. Tannir
  • P. Andrew Futreal
  • Pavlos Msaouel
  • Renzo G. DiNatale
  • Rosalba Minelli
  • Ruohan Xia
  • Sabina Signoretti
  • Samra Turajlic
  • Sanjana Srinivasan
  • Sebastian Lundgren
  • Tim Heffernan
  • Toni K Choueiri
  • Tony Gutschner
  • Truong Nguyen Anh Lam
  • Virginia Giuliani
  • Ying-Bei Chen
  • Ziad Bakouny

Organizations

  • National Cancer Institute
  • The University of Texas MD Anderson Cancer Center
  • United States Department of Defense

Tags

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology
  • Molecular and genetic basis of cancer.

Technology Areas

  • Biotechnology