Krebs cycle metabolon formation: metabolite concentration gradient enhanced compartmentation of sequential enzymes
Abstract
The substrate (l-malate) gradient created in a microfluidic channel induced the directional diffusion of mitochondrial malate dehydrogenase (mMDH) toward a higher concentration region andin situgeneration of an intermediate (OAA) gradient enhanced the co-diffusion of citrate synthase (CS) together with mMDH.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2015
- Source ID
- 10.1039/c4cc08702j
Entities
People
- Fei Wu
- Lindsey N. Pelster
- Shelley D. Minteer
Organizations
- Air Force Office of Scientific Research
- National Science Foundation
- United States Army
- University of Utah
- Yusuf Hamied Department of Chemistry