A universal method for sensitive and cell-free detection of CRISPR-associated nucleases

Abstract

We report a universal assay for sensitive, cell-free detection of CRISPR-associated nucleases by coupling activity to a fluorescent transcription product.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 01, 2019
Source ID
10.1039/c8sc03426e

Entities

People

  • Amit Choudhary
  • Christian Cuba Samaniego
  • Elisa Franco
  • Hari K K Subramanian
  • Kurt J Cox

Organizations

  • Army Research Office
  • Broad Institute
  • Burroughs Wellcome Fund
  • Defense Advanced Research Projects Agency
  • Department of Energy and Climate Change
  • National Institutes of Health
  • United States Army
  • University of California, Riverside
  • University of Cambridge

Tags

Fields of Study

  • Biology

Technology Areas

  • Biotechnology