Spatially isolated reactions in a complex array: using magnetic beads to purify and quantify nucleic acids with digital and quantitative real-time PCR in thousands of parallel microwells

Abstract

Encoded beads carrying primer pairs for nucleic acid targets are used for sample preparation and multiplexed-in-space digital PCR quantification.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 01, 2020
Source ID
10.1039/d0lc00069h

Entities

People

  • John Michael Ramsey
  • Nathan A. Siegfried
  • W. Hampton Henley

Organizations

  • Defense Advanced Research Projects Agency
  • United States Army
  • University of North Carolina at Chapel Hill
  • Yusuf Hamied Department of Chemistry

Tags

Fields of Study

  • Physics

Readers

  • Computational Fluid Dynamics (CFD)
  • Molecular Genetics

Technology Areas

  • Biotechnology
  • Space