Rapid quantification of microRNA-375 through one-pot primer-generating rolling circle amplification
Abstract
Rolling circle amplification (RCA) is enhanced by a primer-generating nickase for detection of prostate cancer-associated microRNA-375. The method improves the detection limit 61-fold compared to standard RCA.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2022
- Source ID
- 10.1039/d2an00263a
Entities
People
- Andrew M Smith
- Chia-Wei Kuo
- Lucas D Smith
- Manish Kohli
- Siva Nalla
Organizations
- National Institutes of Health
- United States Department of Defense
- University of Illinois Urbana–Champaign
- University of Utah