Positive feedback drives a secondary nonlinear product burst during a biphasic DNA amplification reaction
Abstract
Ultrasensitive DNA Amplification Reaction (UDAR) provides a unique biphasic fluorescence output derived from a second phase switch-like burst in reaction products. We quantified the two main reaction products and present a mathematical model that can reproduce the reaction output, including the nonlinear autocatalytic product burst in the second phase.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2022
- Source ID
- 10.1039/d2an01067d
Entities
People
- Burcu Özay
- Esther E. Stopps
- Shannon D. Murphy
- Stephanie McCalla
- Tomas Gedeon
Organizations
- Congressionally Directed Medical Research Programs
- Division of Chemical, Bioengineering, Environmental, and Transport Systems
- National Institute of General Medical Sciences