Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Abstract
Due to their sensitivity and temporal response, optical microresonators are used extensively in the biosensor arena, particularly in the development of label-free diagnostics and measurement of protein kinetics. In the present letter, we investigate using microcavities to probe molecules within biomimetic membranes. Specifically, a method for self-assembling lipid bilayers on spherical microresonators is developed and the bilayer-nature is verified. Subsequently, the microcavity is used to excite a Cy5-conjugated lipid located within the bilayer while the optical performance of the microcavity is characterized. The emission wavelength of the dye and the optical behavior of the microcavity agree with theoretical predictions.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 04, 2011
- Source ID
- 10.1063/1.3576908
Entities
People
- Andrea Armani
- Hong-seok Choi
- Lindsay M. Freeman
- Noah Malmstadt
- Su Li
- Yasaman Dayani
Organizations
- National Institutes of Health
- National Science Foundation
- Office of Naval Research
- University of Southern California