Multimodal coherent anti-Stokes Raman spectroscopic imaging with a fiber optical parametric oscillator
Abstract
We report on multimodal coherent anti-Stokes Raman scattering (CARS) imaging with a source composed of a femtosecond fiber laser and a photonic crystal fiber (PCF)-based optical parametric oscillator (FOPO). By switching between two PCFs with different zero dispersion wavelengths, a tunable signal beam from the FOPO covering the range from 840 to 930 nm was produced. By combining the femtosecond fiber laser and the FOPO output, simultaneous CARS imaging of a myelin sheath and two-photon excitation fluorescence imaging of a labeled axons in rat spinal cord have been demonstrated at the speed of 20 μs per pixel.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 09, 2011
- Source ID
- 10.1063/1.3589356
Entities
People
- Christiane Goulart
- Delong Zhang
- Huifeng Wei
- Jay E. Sharping
- Ji-Xin Cheng
- Mikhail N Slipchenko
- Su Chen
- Weijun Tong
- Yan-hua Zhai
Organizations
- Air Force Office of Scientific Research
- National Institutes of Health
- Purdue University
- University of California