Microbubble array for on-chip worm processing

Abstract

We present an acoustic non-contact technique for achieving trapping, enrichment, and manipulation of Caenorhabditis elegans using an array of oscillating microbubbles. We characterize the trapping efficiency and enrichment ratio under various flow conditions, and demonstrate a single-worm manipulation mechanism through temporal actuation of bubbles. The reason for oscillating bubbles being versatile in processing worms in a microfluidic environment is due to the complex interactions among acoustic field, microbubbles, fluid flow, and live animals. We explain the operating mechanisms used in our device by the interplay among secondary acoustic radiation force, drag force, and the propulsive force of C. elegans.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 14, 2013
Source ID
10.1063/1.4788677

Entities

People

  • Ali Hashmi
  • Gan Yu
  • Hyuck-jin Kwon
  • Jie Xu
  • Xiaolin Chen
  • Xiaonan Lu
  • Yuhao Xu

Organizations

  • Defense Advanced Research Projects Agency
  • University at Buffalo
  • University of British Columbia
  • Washington State University

Tags

Readers

  • Acoustical Oceanography.
  • Fluid Mechanics and Fluid Dynamics.
  • Integrated Circuit Design and Technology.